Share this Abstract

Rate this Abstract

Login to allow rating

Views

Login to allow views

Options


Abstract Details

Abstract Title

The Stress Resilience Mechanism and Signal Regulation of Regular Aerobic Exercise for Aging Rats in Soleus Apoptosis and Autophagy

Abstract Theme

Physical activity and health

Type Presentation

Poster

Abstract Authors

Presenter Liu Wenfeng - Hunan Normal University (Hunan Provincial Key Laboratory of Physical Fitness and Sports Rehabilitation,The Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education) - CN
Yin Dazhong - Hunan Normal University (Hunan Provincial Key Laboratory of Physical Fitness and Sports Rehabilitation,The Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education) - CN
Tang Changfa - Hunan Normal University (Hunan Provincial Key Laboratory of Physical Fitness and Sports Rehabilitation) - CN
Zhang Bingxia - Hunan Normal University (Hunan Provincial Key Laboratory of Physical Fitness and Sports Rehabilitation) - CN
Wei Xia - Hunan Normal University (Hunan Provincial Key Laboratory of Physical Fitness and Sports Rehabilitation) - CN

Presentation Details

Poster Exhibition Site (Local): Yellow - 40        Date: 1 September        Time: 8am to 7pm        Presenter: liu wenfeng

Abstract Resume

Background:
To explore the stress resilience mechanisms of skeletal muscle fibers in the rheological ageing for preventing senile muscle recession and providing the related research data. 

Methods:
In this study, 6-month-old, 16-month-old and 25-month-old specific pathogen free (SPF) male Sprague-Dawley Rat (SD) rats were divided into young (Y-SED), middle-aged (M-SED) and old-aged (O-SED)
sedentary control group, and the corresponding Y-EX, M-EX and O-EX in the aerobic exercise runner group. The 10-weeks of regular moderate-intensity aerobic exercise intervention was carried out in the
aerobic exercise runner group. TUNEL assay and HE staining was used for observing apoptosis index. Western Blot was performed to screen and verify the related signal pathways for the autophagy and
apoptosis molecular mechanisms.

Results:
1) The stereology of soleus muscle fiber bundle and density can be improved and the SOD expression level was also improved in regular aerobic exercise with aging. 2) Apoptosis was increased in all age
group rats soleus, and It’s showed a increased-trend with increasing rheological properties, and apoptosis index was increased respectively by 7.55% and 20.26% (Ρ < 0.05) and 14.52% (Ρ < 0.05) in all
age rats in regular aerobic exercise. 3) LC - III autophagy gene was reduced with the age trend in each sedentary group, but LC - III were significantly increased (Ρ < 0.01) in regular aerobic
exercise all age rats, LC - III were increased with age-trend in each age group. 4) Compared with the corresponding control groups, the expression of autophagy factor Beclin1 were significantly
increased (Ρ < 0.05) in regular aerobic exercise in all age rats, in which the Y - EX was increased the significantly most significant (Ρ < 0.01), but the expression level of Beclin1in each age group
was presented with decreasing trend. 5) Compared with Y - SED group, the expression level of PGC-1 alpha and CaMK II alpha in M - SED group were increased, but the expression level of PGC-1 alpha and
alpha CaMK II in O - SED group was decreased more significantly (Ρ < 0.01) than in the two groups of Y - SED and M - SED. Compared with the sedentary control group, the expression level PGC-1 alpha
was increased in the group Y - EX and M - EX, but the expression level of CaMK II alpha was decreased significantly. but compared with O - SED group, the expression level of PGC-1 alpha and CaMK II
alpha were considerably increased (Ρ < 0.01, Ρ < 0.05).

Conclusions:
1) Cell autophagy and apoptosis in the soleus were changed in regular aerobic exercise in age rats trend and the balance and stability of cell autophagy and apoptosis may influence the development of
aging.2) The balance and stability of cell autophagy and apoptosis may be involved in regulating and adjusting by stimulating the expression of PGC-1 alpha and CaMK II alpha signaling pathways in the
regular aerobic exercise. It is conducive to the soleus biological function and improve skeletal muscle ageing.

Comment this abstract (0 comments)