The imbalance of the Th1/Th2 ratio, which is attributed to exhaustive exercise, can cause an imbalance in the immune function of athletes. Thus, researchers should use special drugs to recover the
Th1/Th2 ratio of athletes that are prone to exhaustion.Exhaustive exercise can impair immune balance in the rat’s body due to change of Th1- and Th2-related cytokines, and some natural products can
regulate immune banlance. The aim of this study is to evaluate the immunoregulation action of cordyceps militaris cordycepin, flammulina velutipes polysaccharide, and glutamine on rats that were
subjected to an intense running session by analyzing Th1- and Th2-related cytokines and transcription factors.
All rats were subjected to the exercise protocol except for a subgroup rat that was used as the negative control group. On the tenth week, the exercise was stopped. All animals were killed by
decapitation after having recovered on the tenth week. Then their blood indexes were analyzed. The mRNA expression of the transcription factors and cytokines in the spleen of rats were assessed via
real-time PCR. Meanwhile, the protein expression of the transcription factors and cytokines in whole blood of rats was determined using ELISA kits and western blotting.
The serum analysis results show that cordycepin showed a remarkable ability in regulating RBC, Hb, and PCV. The induction of leukocyte by polysaccharides was remarkable. The mRNA or protein
expressions of IL-4, GATA3, T-Bet, IFN-γ, STAT1, and STAT6 in the exhaustive exercise subgroup remained higher than those in the CK subgroup, whereas the expression levels of IL-4, GATA3, T-Bet,
IFN-γ, STAT1, and STAT6 in the cordycepin, polysaccharide, and glutamine subgroups were already close to those of the CK subgroup. The results show that cordycepin, polysaccharide, and glutamine could
all induce the expression of the transcription factors and cytokines into normal levels, whereas the effect of polysaccharide and glutamine inducing these transcription factors or cytokines expression
is most significant.
The findings of this study imply that the action of cordycepin had the most significant effect on the immunoregulation of rats that were subjected to exhaustive exercise.