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Abstract Details

Abstract Title

Influence of Hypoxia Exercise on AMPK-PGC-1α in Skeletal Muscle of Rats with Alimentary Obesity

Abstract Theme

Neuroscience and sport

Type Presentation

Poster

Abstract Authors

Presenter Lin Wentao - Guangzhou Sport University (Department of Sports and Health) - CN
Wei Feng - Guangzhou Sport University (Journal Editorial Department) - CN
Zhengyu Zhu - Guangzhou Sport University (Department of Physical Education &Training) - CN
Hongxiang Xu - Guangzhou Sport University (Department of International Exchange) - CN
Juhua Wu - Shanghai University of Sport (School of Sport Science) - CN

Presentation Details

Poster Exhibition Site (Local): Orange - 15        Date: 1 September        Time: 8am to 7pm        Presenter: Lin wentao

Abstract Resume

Background:To explore the effect of hypoxia exercise on the expression levels of AMPK-PGC-1α in skeletal muscle of obese rats. 
Methods:After the alimentary obesity ICR rats modle construction, they were randomly divided into 6 groups(N=10):Nomoxic group(NHQ), Nomoxic group combined with endurance
exercise(NHE), 16.3% hypoxia group(HGQ1), 16.3% hypoxia group combined with endurance exercise(HGE1),13.3% hypoxia group(HGQ2), 13.3% hypoxia group combined with endurance exercise(HGE2); and they
continued to receive high-fat diet. The groups with exercise underwent treading running for 8 weeks, going with 20m/min, 40min/d,5d/w. After 24h later of the last exercise, the blood of rats were
collected.Then leptin and Adiponectin were detected by ELISA. We also detected the protein levels of PGC-1α,pAMPK by Western blot and immunohistochemistry.
Results:1. Alimentary obesity model is constructed successfully after 7 weeks high-diet feeding. 2.After the intervention of hypoxia exercise, the levels of leptin of different
hypoxia exercise groups increase significantly(P<0.01),and the levels of adiponectin of different hypoxia exercise groups decrease (P<0.01 or P<0.05). 3. The phosphorylation of AMPK of HGE1 group in
the skeletal muscle is higher than NHQ group(P<0.05).Compared with NHQ group, NHE group,HGQ1 group, The phosphorylation of AMPK of HGE2 group in the skeletal muscle is higher than NHQ
group(P<0.01).Compared with NHQ group, the expression of PGC-1α of HGE2 group in the skeletal muscle increase significantly(P<0.01),while the expression of PGC-1α of HGE1 group and NHE group in the
skeletal muscle increase slightly(P<0.05). Compared with NHE group, the expression of PGC-1α of HGE2 group in the skeletal muscle increase (P<0.05), but the NHQ group is lower than it. Compared with
HGQ1 group, the expression of PGC-1α of HGE2 group and HGE1 group in the skeletal muscle both increase (P<0.01 or P<0.05). 4. Compared with NHQ group and HGQ1 group, the positive staining of pAMPK in
the skeletal muscle of the rats of NHE group,HGE1 group,HGE2 group are higher than them signifantly (P<0.01); Compared with NHE group, the positive staining  of pAMPK in the skeletal muscle of the
rats of NHQ group and HGQ1 group are lower than it signifantly (P<0.01).Compared with NHQ group,HGQ1 group  and NHE group, the positive staining of pAMPK in the skeletal muscle of the rats of HGE2
group is up-regulated signifantly (P<0.01 or P<0.05); Compared with NHQ group, the positive staining of pAMPK in the skeletal muscle of the rats of HGE1 group is up-regulated signifantly (P<0.05).
Conclusions:1.Hypoxia exercise improves blood lipid metabolism,13.3% hypoxia exercise intervention is better for this subject. 2. Hypoxia exercise may up-regulate the expression 
PGC-1α to improve the blood lipid metabolism by enhancing AMPK phosphorylation.


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