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Abstract Details

Abstract Title

Time-dependent Expression of IL-6, Irisin and Bdnf in Response to Exercise in Rats

Abstract Theme

Physical activity and health

Type Presentation

Oral presentation

Abstract Authors

Presenter Tao Yu - China Institute of Sport Science, CISS (Sport Health and Rehabilitation Center) - CN
Guo Liang Fang - China Institute of Sport Science, CISS (Biology Research Center) - CN
Feng Fei Li - China Institute of Sport Science, CISS (Laboratory Center) - CN
Liang Li - China Institute of Sport Science, CISS (Youth Sport Research Center) - CN
Xing Ya Yang - China Institute of Sport Science, CISS (Laboratory Center) - CN
Yue Meng Wen - China Institute of Sport Science, CISS (Youth Sport Research Center) - CN
Yan Guo - China Institute of Sport Science, CISS (Youth Sport Research Center) - CN
Zi Hong He - China Institute of Sport Science, CISS (Biology Research Center) - CN
Jie Xiu Zhao - China Institute of Sport Science, CISS (Biology Research Center) - CN
Ye Tian - China Institute of Sport Science, CISS (Biology Research Center) - CN

Presentation Details

Room: Marte        Date: 4 September        Time: 17:40:00        Presenter: Tao Yu

Abstract Resume

Background: Irisin, Bdnf and IL-6 Are Exercise-induced Myokines Which Exert Their Effects in Driving Brown-Fat-Like Development (Irisin) and Regulating Fat Oxidation. However, Their
Dynamic Expression in Response to Exercise Is Not Fully Known. Here We Examined the Level of Irisin, Bdnf and IL-6 As Well As the Activation of AMPK and Akt at Different Time Points following
Exercise, in Order to Explore the Pattern of Their Expression and Biological Functions.

Methods: Sprague-Dawley Rats Were Subjected to Downhill Running at 17m/Min for 90 Minutes. Serum Creatine Kinase (CK) Activity Was Tested at Day 1, 3, 5, 7, and 14 following Exercise
to Evaluate Skeletal Muscle Injury. the Serum Level and Skeletal Expression of Bdnf, IL-6 and Irisin Were Measured by Elisa and Real-Time RT-PCR, Respectively. The Activation of AMPK and Akt Signaling
Pathway Was Confirmed by Western Blot. To Compare the Difference Between Two Groups, T-Test Was Employed using SPSS Software and p < 0.05 Was Considered Statistically Significant.

Results: An Increase of CK Activity Was Observed at Day 3 and Day 5 following Exercise, Confirming Exercise-Related Skeletal Muscle Injury. We Didn’t Find Any Increase of IL-6 in the
Rats following Exercise Compared to Sedentary Group, Although It Is Reported that IL-6 Level Is Elevated Dramatically during Exercise. The Level of Irisin and Bdnf Was Increased Both in the Serum and
in the Skeletal Muscle in Day 1 and Day 3 following Downhill Running; Then, While Irisin Level Returned to the Baseline, Serum Bdnf Level Was Continuously Elevated. Besides, AMPK Activation Was Found
at Day 1 and Day 3, but Akt Was Kept Activated Over All the Time Points.

Conclusions: Although IL-6, Irisin and Bdnf Are All Exercise-Induced Myokines, Their Expression Is Regulated Differently. Irisin and Bdnf Are Both Reported to Be Involved in the
Activation of AMPK Signaling Pathway. However, We Found That the Increase of Irisin Was in Accordance with AMPK Activation, While Bdnf Increase Is in Accordance with Akt Activation. Our Results
Suggest that Bdnf May Participate in Skeletal Muscle Regeneration via Akt-Mediated Pathway in Addition to Fat Oxidation. Therefore, Exercise-Induced Myokines Are Regulated Precisely and Sequentially,
Which in Turn Exert Different Functions at Different Time following Exercise.


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