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Expression and Activation of Intracellular Signaling Proteins in Cerebral Cortex and Hippocampal Formation of Aged Rats Submitted to Aerobic or Resistance Exercise.
Neuroscience and sport
Presenter Jessica Salles Henrique - Universidade Federal de São Paulo (UNIFESP) (Fisiologia) - BR
Erivelton Fernandes França - Universidade de Mogi das Cruzes (UMC) (Núcleo de Pesquisa Tecnológica) - BR
Fabrizio dos Santos Cardoso - Universidade de Mogi das Cruzes (UMC) (Núcleo de Pesquisa Tecnológica) - BR
Fernando Tadeu Serra - Universidade de Mogi das Cruzes (UMC) (Núcleo de Pesquisa Tecnológica) - BR
Angélica Begatti Victorino - Universidade Federal de São Paulo (UNIFESP) (Fisiologia) - BR
Alexandre Aparecido de Almeida - Universidade Federal de São Paulo (UNIFESP) (Fisiologia) - BR
Andrea Dominguez Carvalho - Universidade Federal de São Paulo (UNIFESP) (Fisiologia) - BR
Ricardo Mario Arida - Universidade Federal de São Paulo (UNIFESP) (Fisiologia) - BR
Sérgio Gomes da Silva - Universidade de Mogi das Cruzes (UMC) / Hospital Israelita Albert Einstein (HIAE) (Núcleo de Pesquisa Tecnológica / Instituto do Cérebro) - BR
Poster Exhibition Site (Local): Orange - 4 Date: 1 September Time: 8am to 7pm Presenter: Jessica Salles Henrique
Background: Cognitive decline, memory impairment and high susceptibility to neurodegenerative diseases are usually observed in aging. It has been suggested that alterations in intracellular signaling pathways related to proliferation/survival and cell death may be associated to these age-related effects. Thus, pharmacological and non-pharmacological interventions targeting aging-altered intracellular signaling pathways have been indicated for therapeutic purposes. Physical exercise may be a potential intervention. In this way, we performed an experimental study to evaluate cortical and hippocampal expression and activation of intracellular proteins linked to cell proliferation, survival and death (ERK, Akt, p70S6k, CREB, JNK, NF-κB, p38, e STAT-5) in aged rats submitted to two types of exercise (aerobic or resistance training). Methods: Male Wistar rats (n=18; age: 20 months) were distributed in three groups: aerobic training (AER), resistance training (RES) and control (CTL) (n=6 in each group). Aged rats from AER group performed exercise on a treadmill and those from RES were submitted to exercise on a vertical ladder. Both groups were exercised for 8 weeks, 5 times/week, with intensity from 50 to 75% of maximum velocity or load supported. Twenty-four hours after the last training session, the cerebral cortex and hippocampal formation from AER, RES and CTL groups were extracted to analyze the levels of signaling proteins by Luminex MAGPIX system. Results: The aged rats from AER showed a significant increase in cortical expression of total JNK (p=0.028; p=0.024), ERK (p=0.036; p=0.012), Akt (p=0.090; p=0.009), and p70S6K (p=0.005; p=0.004) in relation to aged rats from RES and CTL groups. No significant difference in expression and activation of signaling proteins was found in hippocampal formation of studied groups. Conclusions: Aerobic training in aging results in changes in brain expression of signaling proteins linked to cell proliferation and survival (ERK, Akt and p70S6k) and also death (JNK), mainly in cerebral cortex.