Background:To explore the impact of exercise on the striatal in the early neurodegeneration and its biochemical and molecular regulation mechanism, and to commit to screening the
early new targets of neurodegeneration.
In this study, 6-month-old, 16-month-old and 25-month-old specific pathogen free (SPF) male SD rats were divided into Y-SED, M-SED and O-SED sedentary group, and the corresponding Y-EX, M-EX and O-EX
in the aerobic exercise runner group. The miRNA microarray of miRNA miRCURYTM LNA Array (v.18.0) was deteced to excavate the importance of specific mechanisms of transcription and translation in this
project. qRT-PCR was performed to screen and verify the related signal pathways for the biochemical mechanisms.
miRNA microarray data showed that 26 miRNAs changed two times-fold up or down the level. After screening of the bioinformatics, the expression of miRNA207 up and miRNA542 down by Aerobic Exercise. The
function of miRNA which was related to aging or neurodegenerative of the nervous system has a close relationship with the receptor interaction pathway of nerve stimulation, calcineurin signaling
pathway, γ- aminobutyric acid synaptic signaling pathway and glycolysis signaling pathways.The downstream gene of miRNA on rat striatum in aerobic exercise with the age-aging showed that: Compared
with the corresponding sedentary control group, the expression of CaMKIIα and Vdac1 in the aerobic exercise runner group was increased significantly (Ρ<0.05), which the expression upregulation had a
very significant in M-EX than M-SED (Ρ<0.01). Compared with M-SED and O-SED, the expression of PI3K, Akt and mTOR was upregulated in the corresponding M-EX and O-EX, which compared with O-SED, the
expression of PI3K, Akt and mTOR in O-EX was increased significantly (Ρ<0.01).
The expression of miRNA207 was upregulated and miRNA542 was downregulated, then CaMK and PI3K / Akt / mTOR signaling pathway was activated for regulating on common.