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Abstract Details

Abstract Title

CENTRAL AND PERIPHERAL EEFCTS OF EXERCISE WITHOUT WEIGHT REDUCTION IN OBESE AND LEAN MICE

Abstract Theme

Physical activity and health

Type Presentation

Oral presentation

Abstract Authors

Presenter Francine Pereira de Carvalho - Federal University of São Paulo (Bioscience) - BR
Izabelle Dias Benfato - Federal University of São Paulo (Bioscience) - BR
Thais Ludmilla Moretto - Federal University of São Paulo (Bioscience) - BR
Marcela Barthichoto - Federal University of São Paulo (Bioscience) - BR
Camila Aparecida Machado de Oliveira - Federal University of São Paulo (Bioscience) - BR

Presentation Details

Room: Marte        Date: 4 September        Time: 15:00:00        Presenter: Francine de Carvalho

Abstract Resume

Exercise is one of the most used strategies to fight obesity, but the results are not rare disappointing.  On the other hand, sedentary behavior increases the risk for metabolic and cardiovascular
disease. Thus, despite the reduction in body weight is a desirable outcome a shift in focus from weight loss to improvements in exercise and also diet has been proposed to manage obesity. Wheel
running is frequently used to study the effects of exercise in mice and usually is accompanied by no change in body weight. We took advantage of this to investigate the central and peripheral effects
of exercise (free access to wheel running 5 days/week) on insulin and leptin sensitivity and glucose homeostasis in mice fed a high fat diet for 10 weeks. Male C57BL/6 mice were divided into Control
(C), Control Exercise (CE), High-fat (H) or High-fat Exercise (HE) groups. Glucose, insulin and leptin tolerance, hypothalamic and liver protein expression of components of the insulin and leptin
signaling pathway (by western blotting), plasma leptin, insulin and adiponectin (by ELISA) and body composition were evaluated at the end of the study. Results were analyzed by two-way ANOVA. High fat
diet increased body weight gain with no effect of exercise (H and HE > C and CE). High fat diet also increased perigonadal and retroperitoneal fat depots weight. However, exercise reduced the weight
of both fat depots and decreased the lipid/protein carcass ratio. Although high-fat diet induced glucose intolerance (higher AUC of blood glucose during ipGTT in H and HE compared to C and CE) without
effect of exercise, insulin sensitivity was reduced only in the H group (higher AUC of blood glucose during ipITT in H compared to the other groups). Wheel running increased liver p-Akt expression (CE
and HE > C and H), indicating the insulin action in this organ key to glucose homeostasis was improved by exercise. Consistent with the improved body composition, plasma leptin was higher in the H and
HE compared to C and CE groups, but it was reduced in the exercised (CE and HE) compared to the non-exercised (C and H) groups. The concentration of adiponectin was higher in CE and HE compared to C
and H, with no effect of high fat diet. Plasma insulin was higher in the H and HE groups compared to C and CE groups, with no effect of exercise. This result indicate insulin sensitivity was not fully
restored by exercise. The inhibitory effect of leptin on food intake during the leptin tolerance test was similar among the groups. Accordingly, hypothalamic expressions of total Stat3 and p-Stat3
were affected neither by diet nor by exercise. Expression of total Akt, p-Akt, p-IKK and BDNF in hypothalamus was also similar among the groups. We conclude that despite no change in body weight
exercise has important beneficial effects as it improved body composition, insulin sensitivity and decreased the concentration of a pro-inflammatory hormone (leptin) at the same time it increased the
concentration of an anti-inflammatory hormone (adiponectin). Thus, despite reducing body weight can bring additional benefits, the effects of exercise must not be overlooked when body weight reduction
is not achieved.

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